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Literature summary for 3.2.1.39 extracted from
- Isolation and characterization of two types of beta-1,3-glucanases from the common sea hare Aplysia kurodai (2010), Comp. Biochem. Physiol. B, 155, 138-144.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Aplysia kurodai |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aplysia kurodai | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
x * 36000, SDS-PAGE | Aplysia kurodai |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aplysia kurodai | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 126fold from digestive fluid by ammonium sulfate fractionation, three steps of cation exchange chromatography, ultrafiltration and gel filtration | Aplysia kurodai |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| laminariheptaose + 3 H2O = laminaritriose + 2 laminaribiose | AkLam36 mode of action, overview | Aplysia kurodai |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| digestive juice | - |
Aplysia kurodai | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 172 | - |
purified native enzyme | Aplysia kurodai |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| laminarin + H2O | - |
Aplysia kurodai | laminaribiose + beta-D-glucose | - |
? |  |
| laminarioligosaccharide + H2O | e.g. laminaritetraose | Aplysia kurodai | laminaribiose + beta-D-glucose | - |
? | |
| laminaritetraose + H2O | - |
Aplysia kurodai | ? | - |
? | |
| additional information | AkLam36 specific for beta-1,3-glucosyl linkages. AkLam36 is an endolytic enzyme degrading laminarin and laminarioligosaccharides to laminaritriose, laminaribiose, and D-glucose. AkLam36 shows higher activity toward beta-1,3-glucans with a few beta-1,6-linked glucose branches such as Laminaria digitata laminarin than highly branched beta-1,3-glucans such as Eisenia bicyclis laminarin | Aplysia kurodai | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36000, SDS-PAGE | Aplysia kurodai |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AkLam36 | - |
Aplysia kurodai |
| beta-1,3-glucanase | - |
Aplysia kurodai |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
laminaritetraose | - |
Aplysia kurodai | |
| 23.01 | - |
Laminarin | - |
Aplysia kurodai | |
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Release 2023.1 (January 2023)
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